首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠环加氧酶2(COX-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PTGS2含量。PTGS2，也称为COX-2，是一种在炎症反应和细胞凋亡中起关键调节作用的酶。其研究机制涉及蛋白表达水平和定位，以及与多种疾病如肿瘤、炎症性疾病和心血管疾病的相关性。PTGS2在基因表达调控和组织分布上存在差异，其异常调控与癌症进展和心血管疾病风险增加有关。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，为炎症机制研究、抗炎药物开发、肿瘤微环境分析及神经退行性疾病模型评估等科研领域提供标准化检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ptgs2 ELISA Kit; Cox-2 ELISA Kit; Cox2 ELISA Kit; Pghs-b ELISA Kit; Tis10 ELISA Kit; Prostaglandin G/H synthase 2 ELISA Kit; EC 1.14.99.1 ELISA Kit; Cyclooxygenase-2 ELISA Kit; COX-2 ELISA Kit; Glucocorticoid-regulated inflammatory cyclooxygenase ELISA Kit; Gripghs ELISA Kit; Macrophage activation-associated marker protein P71/73 ELISA Kit; PES-2 ELISA Kit; PHS II ELISA Kit; Prostaglandin H2 synthase 2 ELISA Kit; PGH synthase 2 ELISA Kit; PGHS-2 ELISA Kit; Prostaglandin-endoperoxide synthase 2 ELISA Kit; TIS10 protein ELISA Kit
缩写：PTGS2
Uniprot No.：Q05769
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse COX-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of mouse COX-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.6602.6542.6572.54710002.1072.0461.9941.8845001.4941.3851.4401.3302500.9320.9110.9220.8121250.5020.4890.4960.38662.50.4030.3980.4150.30531.250.2230.2070.2150.10500.1140.1060.110 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse COX-2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled COX-2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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