首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠细胞凋亡相关斑点样蛋白(PYCARD)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PYCARD含量。PYCARD即凋亡相关斑点样蛋白含CARD结构域，在炎性小体激活中起重要作用。它能连接不同蛋白形成复合物，引发炎症反应，参与机体免疫防御。其功能异常与多种疾病如自身免疫病、炎症性疾病相关，因此是药物研发等研究的重要靶点。试剂盒检测范围为7.81 pg/mL-500 pg/mL，适用于体外研究PYCARD在炎症反应、细胞焦亡调控中的动态变化，或用于评估疾病模型中（如脓毒症、关节炎等）PYCARD蛋白的表达水平，为探索免疫调控机制及药物靶点筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pycard ELISA kit; AscApoptosis-associated speck-like protein containing a CARD ELISA kit; mASC ELISA kit; PYD and CARD domain-containing protein ELISA kit
缩写：PYCARD
Uniprot No.：Q9EPB4
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：7.81 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PYCARD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 103 | |||||
| Range % | 95-107 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 87-99 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 86-100 | ||||||
| 1:8 | Average % | 107 | |||||
| Range % | 96-111 | ||||||
回收率：
| The recovery of mouse PYCARD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 86-99 | |||||
| EDTA plasma (n=4) | 103 | 95-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5002.1682.2372.2032.0422501.5221.5041.5131.3521251.0001.0201.0100.84962.50.6440.6820.6630.50231.250.4760.5020.4890.32815.620.3900.4070.3990.2387.810.2670.2590.2630.10200.1590.1630.161 | |||||||
货期：3-5 working days
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