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产品描述：小鼠视黄醇结合蛋白(RBP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的RBP4含量。RBP4即视黄醇结合蛋白4，是一种在肝脏合成并分泌至血液的载体蛋白。它参与视黄醇的转运和代谢过程。研究发现其异常表达与多种疾病如糖尿病、心血管疾病等相关，机制可能包括影响胰岛素敏感性、脂肪代谢等。试剂盒检测范围为0.312 μg/mL-20 μg/mL，为研究维生素A代谢通路、构建相关疾病动物模型或评估药物干预效果提供可靠的检测工具，可满足基础科研中对RBP动态变化的定量监测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Rbp4 ELISA Kit; Retinol-binding protein 4 ELISA Kit; Plasma retinol-binding protein ELISA Kit; PRBP ELISA Kit; RBP ELISA Kit
缩写：RBP4
Uniprot No.：Q00724
种属：Mus musculus (Mouse)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：0.312 μg/mL-20 μg/mL
灵敏度：0.078 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse RBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 86-96 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 90-103 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 85-93 | ||||||
| 1:8 | Average % | 87 | |||||
| Range % | 83-92 | ||||||
回收率：
| The recovery of mouse RBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 93-104 | |||||
| EDTA plasma (n=4) | 94 | 90-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| μg/mlOD1OD2Average 200.1570.1450.151 100.2590.2640.262 50.3690.3850.377 2.50.5870.5450.566 1.250.8300.8230.827 0.6251.2781.2331.256 0.3121.6461.6961.671 02.6222.7452.684 | |||||||
货期：3-5 working days
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