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产品描述：小鼠网状蛋白-4(RTN4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的RTN4含量。RTN4即网状蛋白4，在神经系统等有表达。其背景与细胞内膜系统构建等相关。研究机制上，它参与调节细胞内运输、凋亡等过程，还和神经再生抑制有关，在神经损伤修复、神经系统疾病等方面是潜在研究靶点。试剂盒检测范围为46.88 pg/mL-3000 pg/mL，适用于神经科学领域的基础研究，如脊髓损伤模型评估、脱髓鞘疾病机制探索或神经再生相关药物开发等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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缩写：RTN4
Uniprot No.：Q9NQC3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：46.88 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human RTN4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||
| Sample | Serum(n=4) | |||
| 1:1 | Average % | 97 | ||
| Range % | 92-101 | |||
| 1:2 | Average % | 92 | ||
| Range % | 88-96 | |||
| 1:4 | Average % | 89 | ||
| Range % | 84-93 | |||
| 1:8 | Average % | 96 | ||
| Range % | 91-100 |
回收率：
| The recovery of human RTN4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 99-108 | |||||
| EDTA plasma (n=4) | 84 | 80-88 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 30002.6642.7282.6962.502 15002.1201.9942.0571.863 7501.2001.2511.2261.032 3750.7530.7850.7690.575 187.50.4390.4620.4510.257 93.750.3150.3070.3110.117 46.880.2470.2390.2430.049 00.1910.1960.194 | |||||||
货期：3-5 working days
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