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产品描述：猪基质细胞衍生因子1a(SDF-1a/CXCL12)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的SDF1a/CXCL12含量。SDF1a/CXCL12 是重要靶点。它作为趋化因子，在细胞迁移、增殖等生理过程起关键作用。在研究机制上，其通过与受体结合，激活下游信号通路。在肿瘤、炎症等疾病中也有异常表达和功能，是药物研发潜在方向。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外培养细胞模型、动物实验组织样本或生物体液中的SDF - 1a动态监测；为探索其在心血管疾病、再生医学或免疫微环境调控中的功能机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：SDF1a/CXCL12
种属：Sus scrofa (Pig)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell Lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig SDF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 82-90 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of pig SDF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-102 | ||||
| EDTA plasma (n=4) | 86 | 82-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.8122.7012.7572.564102.3972.3612.3792.18651.7981.7121.7551.5622.51.0110.9790.9950.8021.250.6070.6340.6210.4280.6250.4030.4270.4150.2220.3120.3040.3210.3130.12000.1880.1980.193 | ||||||
货期：3-5 working days
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