首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠臂板蛋白-3D(SEMA3D)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SEMA3D含量。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于研究SEMA3D在神经系统疾病、肿瘤微环境调控或免疫应答机制中的功能，尤其适合探索其在神经轴突导向、细胞迁移及组织再生等生物过程中的分子机制，为神经生物学、免疫学及肿瘤学等基础研究领域提供高效的分析工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sema3d ELISA Kit; Semaphorin-3D ELISA Kit
缩写：SEMA3D
Uniprot No.：Q8BH34
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SEMA3D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 84-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 96-102 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 89-103 | |||||
回收率：
| The recovery of mouse SEMA3D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 80-94 | ||||
| EDTA plasma (n=4) | 98 | 92-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.4332.3322.3832.2247502.0491.9281.9891.8303751.3391.3181.3291.170187.50.7270.7410.7340.575940.4850.4960.4910.332470.2800.2910.2860.12723.50.2210.2250.2230.06400.1590.1580.159 | ||||||
货期：3-5 working days
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