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产品描述：牛硒蛋白P(SEPP1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SEPP1含量。SEPP1即硒蛋白P，在人体硒稳态维持和抗氧化防御等方面发挥重要作用。其研究机制涉及运输硒到各组织，还参与细胞内的抗氧化过程，减轻氧化应激。当前围绕它在神经系统、心血管系统疾病及癌症等方面的作用也有诸多研究。试剂盒检测范围为10 ng/mL-160 ng/mL，该产品可广泛应用于动物营养学研究中的硒元素吸收评估、氧化应激相关疾病模型的生物标志物分析，或环境毒理学中重金属暴露对硒蛋白代谢影响的探索，为畜牧健康管理及基础医学研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SELENOP ELISA Kit; Selenoprotein P ELISA Kit; SeP ELISA Kit; Selenoprotein P-like protein ELISA Kit
缩写：SEPP1
Uniprot No.：P49907
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：10 ng/mL-160 ng/mL
灵敏度：5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine SEPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 89 | |||||
| Range % | 86-102 | ||||||
| 1:200 | Average % | 97 | |||||
| Range % | 91-105 | ||||||
| 1:400 | Average % | 93 | |||||
| Range % | 88-100 | ||||||
| 1:800 | Average % | 91 | |||||
| Range % | 87-96 | ||||||
回收率：
| The recovery of bovine SEPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 86-99 | |||||
| EDTA plasma (n=4) | 93 | 85-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 1600.1050.1020.104 800.1960.1890.193 400.3210.3320.327 200.6220.6080.615 101.0981.1081.103 02.1932.2162.205 | |||||||
货期：3-5 working days
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