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产品描述:小鼠皮质类固醇结合球蛋白(SERPINA6)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆样本中的SERPINA6含量。SERPINA6 作为一种靶点,其与多种生理过程相关。它属于丝氨酸蛋白酶抑制剂家族。目前针对它的研究多聚焦于其在调节蛋白酶活性方面的机制,探索其在疾病发生发展如内分泌紊乱、代谢疾病等进程中的作用及潜在治疗意义。试剂盒检测范围为0.2 μg/mL-80 μg/mL,适用于内分泌机制研究、应激相关疾病模型评估、糖皮质激素代谢动态分析等科研领域;该产品可为探究代谢综合征、免疫紊乱或神经内分泌实验中SERPINA6的表达变化提供有效工具,适用于小鼠体内激素运输功能研究、药物干预对皮质醇结合能力影响评估等基础科研场景本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Serpina6 ELISA kit; CbgCorticosteroid-binding globulin ELISA kit; CBG ELISA kit; Serpin A6 ELISA kit; Transcortin ELISA kit
缩写:SERPINA6
Uniprot No.:Q06770
种属:Mus musculus (Mouse)
样本类型:serum, plasma
检测范围:0.2 μg/mL-80 μg/mL
灵敏度:0.2 μg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<15% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPINA6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 95 | |||||
Range % | 89-102 | ||||||
1:2 | Average % | 95 | |||||
Range % | 87-105 | ||||||
1:4 | Average % | 91 | |||||
Range % | 83-99 | ||||||
1:8 | Average % | 95 | |||||
Range % | 90-100 |
回收率:
The recovery of mouse SERPINA6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 97 | 86-105 | |||||
EDTA plasma (n=4) | 96 | 89-102 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
μg/mlOD1OD2Average02.1352.1212.1280.21.5281.4981.5130.70.9921.0131.0032.50.5440.5520.548150.3390.3520.346800.1600.1570.159 |
货期:3-5 working days
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