首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠纤溶酶原激活物抑制因子1(PAI-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SERPINE1含量。SERPINE1是一个备受关注的靶点。它在多种生理和病理过程中发挥重要作用，如参与凝血、细胞迁移等。研究机制上，多聚焦于其在肿瘤进展、纤维化疾病等中的作用，通过调控相关信号通路来影响疾病发展，为相关疾病治疗提供潜在方向。试剂盒检测范围为0.9 ng/mL-60 ng/mL，适用于心血管疾病模型、血栓形成机制研究以及创伤后组织修复等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Serpine1 ELISA Kit; Mr1 ELISA Kit; Pai1 ELISA Kit; Planh1 ELISA Kit; Plasminogen activator inhibitor 1 ELISA Kit; PAI ELISA Kit; PAI-1 ELISA Kit; Endothelial plasminogen activator inhibitor ELISA Kit; Serpin E1 ELISA Kit
缩写：SERPINE1
Uniprot No.：P22777
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.9 ng/mL-60 ng/mL
灵敏度：0.22 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 88-102 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of mouse PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-101 | ||||
| EDTA plasma (n=4) | 95 | 92-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 603.0333.1083.0712.894 302.4762.5572.5172.340 151.8871.7851.8361.659 7.51.1171.1241.1210.944 3.750.7110.7190.7150.538 1.80.4880.4680.4780.301 0.90.3290.3320.3310.154 00.1780.1760.177 | ||||||
货期：3-5 working days
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