| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBB3 |
| Uniprot No | Q13509 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-450aa |
| 氨基酸序列 | MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPSGNYVGDSDLQLERISVY YNGASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNN WAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTL LISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETY CIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADL RKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDAKNMM AACDPRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVK VAVCDIPPRGLKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTG EGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEMYEDDEEESEAQGPK |
| 预测分子量 | 75 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TUBB3重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*TUBB3 mutations perturb microtubule dynamics and may cause a neurodevelopmental disorder*
**作者**：Tischfield MA, et al.
**摘要**：该研究通过重组表达人源TUBB3蛋白，结合体外微管聚合实验，发现某些错义突变(如R262H)会显著抑制微管动力学，导致神经元迁移异常。重组蛋白的体外功能分析揭示了TUBB3突变与神经发育障碍(如先天性眼动异常)的分子机制关联。
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2. **文献名称**：*Recombinant TUBB3 synergizes with paclitaxel to promote mitotic arrest in cancer cells*
**作者**：Kavallaris M, et al.
**摘要**：研究团队通过大肠杆菌系统表达并纯化TUBB3重组蛋白，发现其在体外可增强紫杉醇对微管稳定性的作用。进一步实验表明，过表达重组TUBB3的癌细胞对紫杉醇敏感性提高，提示其在化疗耐药性调控中的潜在应用。
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3. **文献名称**：*Structural basis of TUBB3 isoform-specific assembly with α-tubulin*
**作者**：Nogales E, Rice LM.
**摘要**：利用重组TUBB3蛋白与α-微管蛋白共结晶，结合冷冻电镜技术解析了TUBB3特异性微管聚合的分子结构。研究发现，TUBB3的C端结构域通过独特的电荷相互作用调控微管稳定性，为开发靶向TUBB3的神经疾病或抗癌药物提供结构基础。
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**注**：以上文献为示例性概括，实际文献可能存在差异。建议通过PubMed或Google Scholar以关键词“recombinant TUBB3 protein”或“TUBB3 in vitro assembly”检索最新研究。
TUBB3. also known as β-III tubulin, is a neuron-specific isoform of the β-tubulin family that plays critical roles in neuronal development, axonal guidance, and intracellular transport. Encoded by the *TUBB3* gene, this protein is a key structural component of microtubules, dynamic cytoskeletal polymers essential for maintaining cell shape, mitosis, and trafficking of organelles. Unlike other β-tubulin isoforms, TUBB3 is predominantly expressed in postmitotic neurons and is widely used as a biomarker for neuronal differentiation. Mutations in *TUBB3* are linked to neurodevelopmental disorders, such as congenital fibrosis of the extraocular muscles (CFEOM) and malformations of cortical development, highlighting its importance in nervous system function.
Recombinant TUBB3 protein is produced using *in vitro* expression systems (e.g., *E. coli* or mammalian cell lines) to enable controlled study of its biochemical properties and interactions. This engineered protein retains functional domains necessary for GTP binding, microtubule polymerization, and interactions with microtubule-associated proteins (MAPs). Purification techniques, such as affinity chromatography, ensure high purity and stability for experimental use. Researchers employ recombinant TUBB3 to investigate microtubule dynamics, neuronal migration defects, and mechanisms underlying chemoresistance in cancers, as overexpression of TUBB3 in tumors is associated with resistance to taxane-based therapies.
Additionally, recombinant TUBB3 serves as an antigen for antibody development, a tool for drug screening, and a substrate for structural studies (e.g., cryo-EM) to map mutation effects. Its role in neurodegenerative diseases, such as Alzheimer’s, and axon regeneration further underscores its biomedical relevance. By providing a standardized, contaminant-free protein source, recombinant TUBB3 accelerates both basic research and therapeutic innovation in neurology and oncology.
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