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产品描述：小鼠血浆蛋白酶C1抑制剂(SERPING1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SERPING1含量。SERPING1是一种编码参与补体级联调节的高度糖基化血浆蛋白的基因，其编码的蛋白质C1抑制剂抑制激活的第一补体成分的C1r和C1s，从而调节补体激活。其缺乏与遗传性血管神经性水肿（HANE）有关，并参与多种疾病的发生。试剂盒检测范围为5.6 ng/mL-360 ng/mL，灵敏度为1.4 ng/mL。适用于基础医学研究中探索免疫调节机制、炎症性疾病模型建立、凝血功能相关实验以及药物干预效果评估等科研场景，为深入解析SERPING1在补体激活、血管通透性调节等生物学过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Serping1 ELISA Kit; C1nh ELISA Kit; Plasma protease C1 inhibitor ELISA Kit; C1 Inh ELISA Kit; C1Inh ELISA Kit; C1 esterase inhibitor ELISA Kit; C1-inhibiting factor ELISA Kit; Serpin G1 ELISA Kit
缩写：SERPING1
Uniprot No.：P97290
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：5.6 ng/mL-360 ng/mL
灵敏度：1.4 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Blood Coagulation
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPING1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:2000 | Average % | 104 | ||||
| Range % | 101-107 | |||||
| 1:4000 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:8000 | Average % | 98 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of mouse SERPING1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 85-92 | ||||
| EDTA plasma (n=4) | 96 | 93-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 3602.5592.5692.5642.461 1802.2532.2982.2762.173 901.8221.8431.8331.730 451.0791.0981.0890.986 22.50.6020.6340.6180.515 11.250.3310.3510.3410.238 5.60.2050.2200.2130.110 00.1020.1040.103 | ||||||
货期：3-5 working days
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