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产品描述：小鼠分泌型卷曲相关蛋白4(SFRP4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SFRP4含量。SFRP4即分泌型卷曲相关蛋白4，它主要对Wnt信号通路进行负调控，在胚胎发育、细胞增殖等过程发挥作用。目前研究发现其表达异常与多种疾病如糖尿病、肿瘤等密切相关，深入探究其机制或为相关疾病治疗提供新靶点。试剂盒检测范围为0.47 ng/mL-30 ng/mL，该产品适用于探索SFRP4在骨骼发育、肿瘤微环境调控、糖尿病相关代谢紊乱等科研场景中的动态表达特征，为构建疾病模型、验证分子机制提供可靠工具，特别适用于需要多类型样本检测的体外实验研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sfrp4 ELISA Kit; Secreted frizzled-related sequence protein 4 ELISA Kit; FRP-4 ELISA Kit; sFRP-4 ELISA Kit
缩写：SFRP4
Uniprot No.：Q9Z1N6
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.47 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SFRP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 84-92 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 107 | ||||
| Range % | 104-110 | |||||
回收率：
| The recovery of mouse SFRP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 89 | 85-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected302.8092.8692.8392.631152.2312.2682.2502.0427.51.5141.5671.5411.3333.750.8450.8000.8230.6151.870.5850.5750.5800.3720.940.4620.4690.4660.2580.470.3420.3440.3430.13500.2090.2070.208 | ||||||
货期：3-5 working days
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