首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肺表面活性物质相关蛋白A(SP-A)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SFTPA1含量。SFTPA1是一个重要靶点。其它在相关生理或病理过程中可能发挥关键作用。研究机制上，目前可能聚焦于它在细胞信号传导、生物合成等方面的功能，探索其与疾病发生发展的联系，以寻找基于该靶点的治疗策略。试剂盒检测范围为3.12 pg/mL-200 pg/mL，尤其适用于动物模型研究中的肺功能评估、呼吸道疾病机制探索以及药物干预效果评价等科研场景，为研究者提供稳定可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sftpa1 ELISA Kit; Sftp-1 ELISA Kit; Sftp1 ELISA Kit; Sftpa ELISA Kit; Pulmonary surfactant-associated protein A ELISA Kit; PSAP ELISA Kit; PSP-A ELISA Kit; SP-A ELISA Kit
缩写：SFTPA1
Uniprot No.：P35242
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2000 | Average % | 96 | ||||
| Range % | 91-105 | |||||
| 1:4000 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:8000 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of mouse SP-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-95 | ||||
| EDTA plasma (n=4) | 93 | 90-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.6372.7142.6762.5491002.3312.3682.3502.223501.8471.9011.8741.747251.2731.2671.2701.14312.50.8450.8690.8570.7306.250.5420.5830.5630.4363.120.3220.3170.3200.19300.1280.1260.127 | ||||||
货期：3-5 working days
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