首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肺表面活性物质相关蛋白B(SP-B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SFTPB含量。小鼠肺表面活性物质相关蛋白B（SP-B）是肺泡表面活性物质的重要组成部分，对降低肺泡表面张力、维持呼吸功能至关重要。研究显示，SP-B的表达与肺损伤、肺部疾病的发生发展密切相关，其基因多态性可能影响肺部疾病的易感性和临床预后。通过构建SP-B转基因小鼠模型，有助于深入探究SP-B在肺部疾病中的作用机制。试剂盒检测范围为0.45 ng/mL-30 ng/mL，本产品适用于基础科研中探究肺部生理病理机制，例如构建急性肺损伤、肺纤维化或新生儿呼吸窘迫综合征等动物模型时，可通过检测SP - B的动态变化评估肺功能状态。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sftpb ELISA Kit; Sftp3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
缩写：SFTPB
Uniprot No.：P50405
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.45 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-94 | |||||
回收率：
| The recovery of mouse SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-96 | ||||
| EDTA plasma (n=4) | 102 | 96-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected302.1762.2062.1912.031151.5111.5441.5281.3687.50.8970.9700.9340.7743.750.6120.6180.6150.4551.80.3620.3920.3770.2170.90.2790.2670.2730.1130.450.2250.2200.2230.06300.1560.1630.160 | ||||||
货期：3-5 working days
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