首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠细胞因子信号转导抑制因子1(SOCS-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SOCS1含量。SOCS1是重要的细胞内负反馈调节因子。它可调控细胞因子信号传导，避免过度免疫反应。在研究机制上，能与受体和激酶结合抑制JAK-STAT通路。其异常表达与肿瘤、自身免疫病等疾病相关，是潜在的药物作用靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于免疫学、炎症相关疾病机制或细胞信号转导等基础研究领域。特别适用于评估小鼠实验模型中SOCS - 1在炎症性疾病、自身免疫反应或肿瘤微环境调控中的表达特征，为探索相关信号通路的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Socs1 ELISA Kit; Cish1 ELISA Kit; Ssi1 ELISA Kit; Suppressor of cytokine signaling 1 ELISA Kit; SOCS-1 ELISA Kit; JAK-binding protein ELISA Kit; JAB ELISA Kit; STAT-induced STAT inhibitor 1 ELISA Kit; SSI-1 ELISA Kit
缩写：SOCS-1
Uniprot No.：O35716
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SOCS-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 84-94 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-102 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of mouse SOCS-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 86-95 | ||||
| EDTA plasma (n=4) | 99 | 94-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.4822.4522.4672.32820001.9762.0712.0241.88510001.6041.6631.6341.4955001.1951.1391.1671.0282500.6240.6140.6190.4801250.4390.4160.4280.28962.50.2290.2460.2380.09900.1410.1370.139 | ||||||
货期：3-5 working days
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