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产品描述：人Kazal型丝氨酸蛋白酶抑制剂5(SPINK5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SPINK5含量。SPINK5基因编码的蛋白参与多种生理过程，其异常与不少疾病相关。在研究机制方面，主要聚焦于它在蛋白酶调控中的作用，当SPINK5功能失调，蛋白酶活性失衡，会影响皮肤屏障功能等，为疾病病理机制及治疗靶点研究提供方向。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于基础医学研究中皮肤屏障功能评估、免疫调控机制探索、炎症性疾病模型构建等领域，为体外实验提供可靠的蛋白定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hemofiltrate peptide HF7665 ELISA Kit; ISK5_HUMAN ELISA Kit; LEKTI ELISA Kit; Lympho-epithelial Kazal-type-related inhibitor ELISA Kit; Lymphoepithelial Kazal type related inhibitor ELISA Kit; NETS ELISA Kit; NS ELISA Kit; Serine peptidase inhibitor Kazal type 5 ELISA Kit; SPINK5 ELISA Kit; VAKTI ELISA Kit
缩写：SPINK5
Uniprot No.：Q9NQ38
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SPINK5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 98-107 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of human SPINK5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-95 | ||||
| EDTA plasma (n=4) | 107 | 99-111 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.4782.4112.4452.31820001.9651.9761.9711.84410001.4521.4771.4651.3385000.9060.8890.8980.7712500.5640.5470.5560.4291250.3430.3340.3390.21262.50.2570.2510.2540.12700.1280.1260.127 | ||||||
货期：3-5 working days
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