首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子可溶性P选择素(sP-selectin)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液、唾液、组织匀浆样本中的sP-selectin含量。sP - selectin是一个受关注的靶点。它在炎症、血栓形成等病理生理过程中发挥重要作用。相关研究主要聚焦其介导细胞粘附、调节免疫反应等机制，通过抑制或调控它的活性，有望为治疗炎症性疾病、心血管疾病等开拓新的方向。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，可满足基础科研中疾病机制研究、药物作用评估或生物标志物探索等需求，尤其适用于炎症模型、血管功能实验或代谢相关研究的体外样本检测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：sP-selectin
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, cell culture supernates, urine, saliva, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit sP-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 98 | ||||
| Range % | 95-101 | |||||
| 1:40 | Average % | 96 | ||||
| Range % | 93-99 | |||||
| 1:80 | Average % | 87 | ||||
| Range % | 85-90 | |||||
| 1:160 | Average % | 92 | ||||
| Range % | 87-95 | |||||
回收率：
| The recovery of rabbit sP-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 93-99 | ||||
| EDTA plasma (n=4) | 101 | 99-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average20000.0900.0920.09110000.1350.1260.1315000.2090.2040.2072500.3190.3170.3181250.4570.4460.45262.50.5810.5980.59031.250.8120.8360.82401.5711.5981.585 | ||||||
货期：3-5 working days
×
