首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人斯钙素-2(STC2)酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的STC2含量。人斯钙素-2（STC2）是一种糖蛋白，广泛表达于多种肿瘤细胞和组织中。其表达受多种应激条件调控，有助于细胞应对应激状态，防止细胞凋亡。STC2过表达与肿瘤生长、侵袭、转移及患者预后相关，被视为一种通用肿瘤生物标志物和潜在治疗靶点。试剂盒检测范围为31.2 pg/mL-2000 pg/mL，适用于体外实验中对STC2表达水平的动态监测，如肿瘤微环境分析、代谢相关信号通路研究、细胞应激模型构建等基础科研领域，为探索STC2在疾病发生发展中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Stanniocalcin 2 ELISA Kit; Stanniocalcin related protein ELISA Kit; Stanniocalcin-2 ELISA Kit; Stanniocalcin-related protein ELISA Kit; Stanniocalcin2 ELISA Kit; STC 2 ELISA Kit; STC related protein ELISA Kit; STC-2 ELISA Kit; STC-related protein ELISA Kit; STC2 ELISA Kit; STC2_HUMAN ELISA Kit; STCRP ELISA Kit
缩写：STC2
Uniprot No.：O76061
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.2 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human STC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
| 1:4 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:8 | Average % | 110 | |||||
| Range % | 104-114 | ||||||
回收率：
| The recovery of human STC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 91-99 | |||||
| EDTA plasma (n=4) | 103 | 98-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.0962.1032.1001.98210001.6411.5981.6201.5025001.1051.2011.1531.0352500.6660.6720.6690.5511250.4340.4420.4380.32062.50.2680.2560.2620.14431.250.2110.2010.2060.08800.1160.1200.118 | |||||||
货期：3-5 working days
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