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产品描述：牛凝血酶抗凝血酶Ⅲ复合物(TAT-III)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的TAT-III含量。试剂盒检测范围为1.56 pg/mL-100 pg/mL，本试剂盒可应用于心血管疾病动物模型研究、凝血相关药物开发中的药效评价，或探索凝血 - 抗凝系统动态平衡的分子调控机制，为科研领域提供灵敏、特异的检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TAT-III
种属：Bos taurus (Bovine)
样本类型：serum, plasma
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine TAT-III in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:20 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:40 | Average % | 85 | |||||
| Range % | 80-90 | ||||||
| 1:80 | Average % | 104 | |||||
| Range % | 100-110 | ||||||
| 1:160 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
回收率：
| The recovery of bovine TAT-III spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 89 | 85-93 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2Average 1000.1590.1630.161 500.2790.2670.273 250.4550.4710.463 12.50.7240.7040.714 6.251.0711.0061.039 3.121.2791.3221.301 1.561.6831.7181.701 02.1062.2352.171 | |||||||
货期：3-5 working days
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