首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠端粒酶(TE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TE含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本试剂盒产品广泛应用于科研领域，包括但不限于端粒酶活性相关的基础机制研究、抗衰老药物筛选、肿瘤细胞增殖模型构建以及再生医学中的干细胞功能评估等实验场景，为探索TE在疾病发生发展中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：TE
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 87 | ||||
| Range % | 82-91 | |||||
| 1:10 | Average % | 104 | ||||
| Range % | 100-108 | |||||
| 1:20 | Average % | 99 | ||||
| Range % | 96-105 | |||||
| 1:40 | Average % | 91 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of mouse TE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 88 | 83-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.4032.4692.4362.28051.4851.4021.4441.2882.50.9590.9130.9360.7801.250.5100.5350.5230.3670.6250.3830.3910.3870.2310.3120.2780.2980.2880.1320.1560.2090.2100.2100.05400.1570.1540.156 | ||||||
货期：3-5 working days
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