首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠转化生长因子α(TGF-α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TGFA含量。TGFA即转化生长因子-α，是表皮生长因子家族成员。它能与表皮生长因子受体结合。在研究机制上，其参与细胞的增殖、分化等过程，在肿瘤发生发展、伤口愈合等方面发挥作用，是潜在的疾病治疗靶点。试剂盒检测范围为12.5 pg/mL-800 pg/mL，该产品适用于体外研究小鼠炎症反应、肿瘤微环境、干细胞分化等模型中TGF-α的动态变化，为探索TGF-α在细胞信号转导与疾病机制中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tgfa ELISA Kit; Protransforming growth factor alpha [Cleaved into: Transforming growth factor alpha ELISA Kit; TGF-alpha ELISA Kit; EGF-like TGF ELISA Kit; ETGF ELISA Kit; TGF type 1)] ELISA Kit
缩写：TGFA
Uniprot No.：P48030
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 96-105 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of mouse TGF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-96 | ||||
| EDTA plasma (n=4) | 92 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8002.8912.8742.8832.7204002.4512.3322.3922.2292001.9911.8871.9391.7761001.3521.3451.3491.186500.7070.6920.7000.537250.4480.4310.4400.27712.50.2880.2680.2780.11500.1650.1610.163 | ||||||
货期：3-5 working days
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