首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠转化生长因子β3(TGF-β3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TGFB3含量。TGFB3即转化生长因子β3，在胚胎发育、组织修复等多种生理过程中扮演重要角色。其研究机制主要围绕调节细胞生长、分化、黏附及凋亡等方面。异常表达与肿瘤发生发展、纤维化疾病等密切相关，对其深入研究有望为相关疾病治疗提供新策略。试剂盒检测范围为125 pg/mL-8000 pg/mL，本试剂盒适用于体外实验研究，可广泛用于探究TGF-β3在细胞功能调控、炎症或纤维化动物模型中的动态变化，亦可用于评估体外培养细胞分泌TGF-β3的能力，为研究组织再生、疾病机制及药物干预效果提供可靠的科研工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tgfb3 ELISA Kit; Transforming growth factor beta-3 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-3 ELISA Kit; TGF-beta-3)] ELISA Kit
缩写：TGFB3
Uniprot No.：P17125
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGF-β3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 93-102 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of mouse TGF-β3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 91 | 84-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.6462.8152.7312.54240002.0162.1552.0861.89720001.1951.2641.2301.04110000.7780.7980.7880.5995000.4930.5060.5000.3112500.3360.3610.3490.1601250.2790.2650.2720.08300.1920.1850.189 | ||||||
货期：3-5 working days
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