首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人酪氨酸羟化酶(TH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TH含量。人酪氨酸羟化酶（TH）是儿茶酚胺生物合成的关键限速酶，催化L-酪氨酸转化为多巴，为多巴胺等神经递质的前体。其表达和功能异常与多种疾病相关，成为研究热点。研究机制涉及TH的调控、基因表达、以及与疾病的关系。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品适用于神经科学领域的基础研究，包括疾病模型构建、药物干预效果评估以及神经递质代谢通路分析，实验全程无需特殊设备，操作流程标准化，重复性良好，可为神经生物学、药理学研究提供可靠的定量检测支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Dystonia 14 ELISA Kit; DYT14 ELISA Kit; DYT5b ELISA Kit; EC 1.14.16.2 ELISA Kit; OTTHUMP00000011225 ELISA Kit; OTTHUMP00000011226 ELISA Kit; ple ELISA Kit; Protein Pale ELISA Kit; TH ELISA Kit; The ELISA Kit; TY3H_HUMAN ELISA Kit; TYH ELISA Kit; Tyrosine 3 hydroxylase ELISA Kit; Tyrosine 3 monooxygenase ELISA Kit; Tyrosine 3-hydroxylase ELISA Kit; Tyrosine 3-monooxygenase ELISA Kit; Tyrosine hydroxylase ELISA Kit
缩写：TH
Uniprot No.：P07101
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 87-103 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 82-97 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 85-105 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-110 | |||||
回收率：
| The recovery of human TH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-96 | ||||
| EDTA plasma (n=4) | 93 | 85-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.5392.4892.5142.398 102.0131.9952.0041.888 51.4971.5421.5201.404 2.51.0901.0271.0590.943 1.250.6760.6610.6690.553 0.6250.4250.4340.4300.314 0.3120.2510.2630.2570.141 00.1140.1180.116 | ||||||
货期：3-5 working days
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