关键信息
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基因名
RACGAP1
- 应用
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别名
HsCYK-4; ID-GAP; MgcRacGAP; Male germ cell RacGap
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种属
Human
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表达系统
E. coli
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标签
N- His & GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H0H5
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表达区间
Met106~Trp539
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分子量
78kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
RACGAP1 (RAC GTPase-activating protein 1) is a critical regulator of the Rho GTPase family, particularly involved in the processes of cell division and cytokinesis. Its primary role is to facilitate the cleavage of RhoA-GTP, leading to the promotion of contractile ring formation during mitosis. Dysregulation of RACGAP1 has been implicated in various cancers, where it contributes to altered cell proliferation and invasion. Research has shown that RACGAP1 not only interacts with other key proteins involved in the mitotic machinery, such as CEP55 and ECT2, but it also plays a role in the signaling pathways that govern cellular responses to external stimuli. The production of recombinant RACGAP1 protein allows for the detailed study of its structural and functional aspects, aiding in elucidating its mechanism of action and its role in developmental processes and disease. Ongoing studies focus on the potential of RACGAP1 as a therapeutic target, highlighting the necessity for deeper understanding of its pathways and interactions in cancer biology and cell cycle regulation. The investigation of this protein's properties can pave the way for novel strategies in cancer treatment and provide insights into the fundamental processes of cell division.
