| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS27A |
| Uniprot No | P62979 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-76aa |
| 氨基酸序列 | MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS27A重组蛋白的3篇参考文献示例(注：文献为虚拟示例，实际引用需检索真实数据库)：
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1. **文献名称**：*Recombinant RPS27A expression in E. coli and its role in ubiquitin-ribosome fusion*
**作者**：Lee, J. et al.
**摘要**：本研究成功在大肠杆菌中表达了重组RPS27A蛋白，并验证其作为泛素前体与核糖体整合的双重功能。纯化后的蛋白通过质谱及体外泛素化实验，证实其参与泛素-蛋白酶体调控通路。
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2. **文献名称**：*Structural analysis of human RPS27A using recombinant protein crystallography*
**作者**：Zhang, Y. & Wang, H.
**摘要**：作者利用昆虫细胞系统表达并纯化重组RPS27A蛋白，通过X射线晶体学解析其三维结构，揭示了其泛素样结构域与核糖体亚基结合的分子机制，为靶向RPS27A的癌症治疗提供依据。
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3. **文献名称**：*Overexpression of RPS27A in hepatocellular carcinoma and functional validation via recombinant protein*
**作者**：Chen, L. et al.
**摘要**：通过构建重组RPS27A稳转肝癌细胞系，发现其过表达显著促进肿瘤细胞增殖。体外实验表明，重组RPS27A通过调控p53泛素化降解影响癌细胞周期。
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如需真实文献，建议在PubMed或Web of Science中检索关键词：“RPS27A recombinant”、“RPS27A ubiquitin expression”等。
RPS27A, also known as ribosomal protein S27a, is a multifunctional component of the 40S ribosomal subunit involved in protein biosynthesis. Beyond its canonical role in translation, RPS27A is unique as a ubiquitin fusion protein. In humans, it is initially synthesized as a C-terminal extension of ubiquitin (Ub-RPS27A), which is post-translationally processed to generate free ubiquitin and mature RPS27A. This dual identity links ribosomal function to ubiquitin-mediated protein degradation pathways, highlighting its regulatory versatility.
Recombinant RPS27A protein is produced using expression systems like *E. coli* or mammalian cells, often with affinity tags (e.g., His-tag) for purification. Its production enables studies on ribosome biogenesis, ubiquitin dynamics, and crosstalk between translation and proteostasis. Structurally, RPS27A contains a conserved zinc-binding domain critical for ribosomal assembly and a ubiquitin-like region that may participate in non-ribosomal interactions.
Research highlights RPS27A's involvement in disease pathways. Dysregulation is observed in cancers (e.g., liver, breast) where it influences cell proliferation and apoptosis via p53 stabilization or NF-κB signaling. In neurodegenerative disorders like Alzheimer’s, altered RPS27A expression correlates with disrupted protein homeostasis. Additionally, its ubiquitin moiety contributes to the cellular free ubiquitin pool, impacting substrate tagging for proteasomal degradation.
Recombinant RPS27A serves as a tool to explore post-translational modifications, ribosome-ubiquitin network crosstalk, and therapeutic targeting. Its study bridges ribosomal biology and ubiquitin-dependent regulatory mechanisms, offering insights into diseases linked to translational or proteostatic dysfunction.
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