Cat: IPD-X38868

Recombinant Human ORNT1 Protein,His

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关键信息

  • 基因名

    ORNT1

  • 应用

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    SLC25A15; HHH; ORC1; Solute Carrier Family 25 Member 15

  • 种属

    Human

  • 表达系统

    E. coli

  • 标签

    N-His

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    Q9Y619

  • 表达区间

    Met1~Tyr301

  • 分子量

    33kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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背景信息

ORNT1 (octanoyltransferase 1) is an enzyme that plays a significant role in mitochondrial fatty acid metabolism and is essential for the biosynthesis of mitochondrial translation factors. Its primary function involves the transfer of acyl groups, particularly octanoyl groups, to specific proteins, which is crucial for the post-translational modification of these proteins and their subsequent function in mitochondrial protein synthesis. Research into ORNT1 has gained momentum due to its implications in various metabolic disorders, mitochondrial dysfunctions, and their associated pathologies. Understanding the structure and function of ORNT1 is vital for elucidating its role in cellular energy metabolism and the regulation of fatty acid synthesis. Furthermore, altered expression or mutations in ORNT1 have been linked to several diseases, including diabetes and cardiomyopathy, highlighting its potential as a therapeutic target. The recombinant production of ORNT1 allows for in-depth studies that can provide insights into its enzymatic mechanisms and interactions with other mitochondrial proteins. This research also paves the way for the development of novel treatments for conditions linked to mitochondrial dysfunction. By utilizing recombinant DNA technology, scientists can produce large quantities of ORNT1, enabling biochemical assays and structural studies that may reveal critical information about its function and regulation in lipid metabolism and overall mitochondrial health. Ultimately, a comprehensive understanding of ORNT1's role could lead to advancements in the treatment of metabolic disorders and better strategies for managing diseases associated with mitochondrial dysfunction.

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