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产品描述：人跨膜蛋白119(TMEM119)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TMEM119含量。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于体外培养的小胶质细胞模型中蛋白表达水平检测、动物脑组织炎症状态评估，或探索外周血中TMEM119与神经系统疾病潜在关联的科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TMEM119 ELISA Kit; PSEC0199 ELISA Kit; UNQ731/PRO1415 ELISA Kit; Transmembrane protein 119 ELISA Kit; Osteoblast induction factor ELISA Kit; OBIF ELISA Kit
缩写：TMEM119
Uniprot No.：Q4V9L6
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TMEM119 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:2 | Average % | 88 | |||||
| Range % | 84-92 | ||||||
| 1:4 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:8 | Average % | 90 | |||||
| Range % | 87-93 | ||||||
回收率：
| The recovery of human TMEM119 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 104 | 100-108 | |||||
| EDTA plasma (n=4) | 88 | 84-93 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40001.8051.8311.8181.69120001.5011.6001.5511.42410001.2821.1981.2401.1135001.0031.0681.0360.9092500.7040.7120.7080.5811250.4710.4980.4850.35862.50.3560.3620.3590.23200.1260.1280.127 | |||||||
货期：3-5 working days
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