首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肿瘤坏死因子α（TNF-α）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TNF含量。TNF（肿瘤坏死因子）是一种细胞因子，在免疫调节和炎症反应中起关键作用。其过度表达与多种疾病如类风湿关节炎、炎症性肠病等相关。研究机制聚焦于TNF与受体结合后激活相关信号通路，引发炎症级联反应，为药物研发提供靶点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于科研场景中炎症机制研究、免疫调控实验、药物筛选模型构建等，可分析大鼠炎症性疾病模型中的TNF-α动态变化、评估感染免疫反应或药物干预效果，其兼容多种生物样本的特点为体外实验与体内研究提供灵活解决方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tnf ELISA Kit; Tnfa ELISA Kit; Tnfsf2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
缩写：TNF
Uniprot No.：P16599
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 97-105 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-107 | |||||
回收率：
| The recovery of rat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 80-92 | ||||
| EDTA plasma (n=4) | 99 | 95-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
 pg/mlOD1OD2AverageCorrected 4002.0372.0672.0521.871 2001.4451.4891.4671.286 1001.0171.0341.0260.845 500.6550.6770.6660.485 250.4110.4380.4250.244 12.50.3510.3780.3650.184 6.250.2970.2820.2900.109 00.1830.1790.181 | ||||||
货期：3-5 working days
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