首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠可溶性核因子κB受体活化因子配基(sRANKL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的TNFSF11含量。TNFSF11是肿瘤坏死因子配体超家族成员11，也叫核因子κB受体活化因子配体（RANKL）。它在骨代谢中调节破骨细胞生成与活化。研究机制主要围绕其与受体结合调节信号通路，还涉及肿瘤骨转移、免疫调节等方面，是抗骨质疏松等药物研发的重要靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，在骨质疏松、关节炎等骨骼疾病研究及免疫系统相关机制探索中具有重要科研价值；适用于体外实验模型中的sRANKL动态监测，例如骨代谢研究、药物干预效果评估、炎症相关细胞因子网络分析等科研场景，为探索骨重塑机制或免疫调控通路提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tnfsf11 ELISA Kit; Opgl ELISA Kit; Rankl ELISA Kit; Trance ELISA Kit; Tumor necrosis factor ligand superfamily member 11 ELISA Kit; Osteoclast differentiation factor ELISA Kit; ODF ELISA Kit; Osteoprotegerin ligand ELISA Kit; OPGL ELISA Kit; Receptor activator of nuclear factor kappa-B ligand ELISA Kit; RANKL ELISA Kit; TNF-related activation-induced cytokine ELISA Kit; TRANCE ELISA Kit; CD antigen CD254) [Cleaved into: Tumor necrosis factor ligand superfamily member 11 ELISA Kit; membrane form; Tumor necrosis factor ligand superfamily member 11 ELISA Kit; soluble form] ELISA Kit
缩写：TNFSF11
Uniprot No.：Q9ESE2
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sRANKL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-101 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 93-102 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-99 | |||||
回收率：
| The recovery of rat sRANKL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-100 | ||||
| EDTA plasma (n=4) | 100 | 96-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40001.9341.8291.8821.71920001.1941.1391.1671.00410000.7530.7330.7430.5805000.5480.5380.5430.3802500.3850.3750.3800.2171250.3040.2940.2990.13662.50.2410.2370.2390.07600.1630.1620.163 | ||||||
货期：3-5 working days
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