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产品描述：人心肌肌钙蛋白Ⅰ(cTn-Ⅰ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液样本中的TNNI3含量。TNNI3是心脏肌钙蛋白 I 的编码基因，其蛋白在心肌收缩与舒张过程中起关键调节作用。研究中，TNNI3变异与心肌病等心血管疾病密切相关，通过解析其致病机制，有望为相关疾病的诊断、治疗及药物研发提供新方向。试剂盒检测范围为47 pg/mL-3000 pg/mL，心血管疾病动物模型评估、药物心脏毒性研究、运动医学中心肌适应性分析等科研场景，生物标志物筛选或疾病机制研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit; cardiomyopathy; dilated 2A (autosomal recessive) ELISA Kit; Cardiomyopathy; familial hypertrophic; 7; included ELISA Kit; CMD1FF ELISA Kit; CMD2A ELISA Kit; CMH7 ELISA Kit; cTnI ELISA Kit; Familial hypertrophic cardiomyopathy 7 ELISA Kit; MGC116817 ELISA Kit; RCM1 ELISA Kit; Tn1 ELISA Kit; Tni ELISA Kit; TNN I3 ELISA Kit; TNNC 1 ELISA Kit; TNNC1 ELISA Kit; TNNI3 ELISA Kit; TNNI3_HUMAN ELISA Kit; Troponin I ELISA Kit; Troponin I cardiac ELISA Kit; Troponin I cardiac muscle ELISA Kit; Troponin I cardiac muscle isoform ELISA Kit; Troponin I type 3 cardiac ELISA Kit; troponin I; cardiac 3 ELISA Kit; TroponinI ELISA Kit; Ttroponin I type 3 (cardiac) ELISA Kit
缩写：TNNI3
Uniprot No.：P19429
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 83-101 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-103 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of human cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 84-110 | ||||
| EDTA plasma (n=4) | 96 | 89-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.3622.4072.3852.23815001.4211.4531.4371.2907500.8520.8460.8490.7023750.4890.5120.5010.354187.50.3230.3360.3300.183940.2800.2860.2830.136470.2280.2270.2280.08100.1460.1470.147 | ||||||
货期：3-5 working days
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