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Human Tryptase beta-2(TPSB2) ELISA kit

  • 中文名： 人胰蛋白酶β-2(TPSB2)酶联免疫试剂盒
货号: IPD-EL024128HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人胰蛋白酶β-2(TPSB2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TPSB2含量。TPSB2（类胰蛋白酶2）是一种胰蛋白酶样丝氨酸蛋白酶，属于肽酶家族S1。它仅作为肝素稳定的四聚体具有酶促活性，对所有已知的内源性蛋白酶抑制剂具有抗性，并在哮喘和其他过敏性和炎症性疾病发病机制中发挥作用。试剂盒检测范围为0.137 ng/ml- 100 ng/ml，支持科研领域对TPSB2的功能研究，例如炎症性疾病模型的分子机制探索、药物干预效果评估或细胞分泌功能分析，也可用于生物样本中TPSB2表达水平的定量比较实验。适用于生命科学基础研究及生物标志物开发等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Mast cell tryptase beta II ELISA Kit; Mast cell tryptase beta III ELISA Kit; TPS2 ELISA Kit; TPSB2 ELISA Kit; TRYB2_HUMAN ELISA Kit; Tryptase beta 2 (gene/pseudogene) ELISA Kit; Tryptase beta-2 ELISA Kit; Tryptase II ELISA Kit; Tryptase-2 ELISA Kit; TryptaseB ELISA Kit; TryptaseC ELISA Kit
  • 缩写：
    TPSB2
  • Uniprot No.：
    P20231
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.137 ng/ml - 100 ng/ml
  • 灵敏度：
    0.137 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human TPSB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    92


    Range %

    85-99


    1:2

    Average %

    89


    Range %

    84-95


    1:4

    Average %

    93


    Range %

    89-98


    1:8

    Average %

    90


    Range %

    83-97


  • 回收率：

    The recovery of human TPSB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    93

    86-100


    EDTA plasma (n=4)

    101

    96-107


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    100

    2.576

    2.634

    2.605

    2.502


    33.33

    1.807

    1.767

    1.787

    1.684


    11.11

    1.124

    1.188

    1.156

    1.053


    3.7

    0.639

    0.629

    0.634

    0.531


    1.23

    0.388

    0.371

    0.380

    0.277


    0.41

    0.296

    0.286

    0.291

    0.188


    0.137

    0.185

    0.184

    0.185

    0.082


    0

    0.101

    0.105

    0.103




  • 货期：
    3-5 working days

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