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产品描述：兔子抗酒石酸酸性磷酸酶5b(TRAP5b)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TRAP5b含量。试剂盒检测范围为78 μU/mL-5000 μU/mL，适用于科研领域中对骨代谢相关疾病（如骨质疏松、骨转移癌、风湿性关节炎等）的机制研究，也可用于药物开发中破骨细胞活性调节剂的体外效果评估。通过检测样本中TRAP5b的表达水平，研究者可量化分析骨吸收活性变化，为骨骼系统疾病模型构建、病理机制探索及治疗策略优化提供实验依据。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ACP5 ELISA Kit; Tartrate-resistant acid phosphatase type 5 ELISA Kit; TR-AP ELISA Kit; EC 3.1.3.2 ELISA Kit; Tartrate-resistant acid ATPase ELISA Kit; TrATPase ELISA Kit; Type 5 acid phosphatase ELISA Kit
缩写：TRAP5b
Uniprot No.：O97860
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：78 μU/mL-5000 μU/mL
灵敏度：19.5 μU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TRAP5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 88-101 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 81-94 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 87-101 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 84-95 | |||||
回收率：
| The recovery of rabbit TRAP5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 84-96 | ||||
| EDTA plasma (n=4) | 103 | 93-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μU/mlOD1OD2AverageCorrected50001.9941.9121.9531.83525001.7761.7451.7611.64312501.5041.5611.5331.4156251.2121.1781.1951.0773120.9290.9000.9150.7971560.5060.4980.5020.384780.2890.2820.2860.16800.1160.1200.118 | ||||||
货期：3-5 working days
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