首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人转化受体电位阳离子通道亚家族V成员1(TrpV1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TRPV1含量。TRPV1 是一种重要的离子通道受体。它广泛分布于感觉神经末梢，在痛觉信号传导等生理过程中起关键作用。研究机制上，其能被多种内源性和外源性物质激活，通过调节钙离子内流等引发生理反应，是开发镇痛等药物的热门靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于探究TrpV1在神经退行性疾病、慢性疼痛模型、炎症反应调控中的分子机制，也可用于药物开发过程中靶向TrpV1的化合物筛选及作用效果评估，为神经科学、药理学及基础医学研究提供标准化检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Capsaicin receptor ELISA Kit; DKFZp434K0220 ELISA Kit; osm 9 like TRP channel 1 ELISA Kit; Osm-9-like TRP channel 1 ELISA Kit; OTRPC1 ELISA Kit; Transient receptor potential cation channel subfamily V member 1 ELISA Kit; TRPV 1 ELISA Kit; Trpv1 ELISA Kit; TRPV1_HUMAN ELISA Kit; Vanilloid receptor 1 ELISA Kit; Vanilloid receptor subtype 1 ELISA Kit; VR 1 ELISA Kit; VR1 ELISA Kit
缩写：TRPV1
Uniprot No.：Q8NER1
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TrpV1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||
| Sample | Serum(n=4) | |||
| 1:1 | Average % | 92 | ||
| Range % | 84-98 | |||
| 1:2 | Average % | 94 | ||
| Range % | 89-103 | |||
| 1:4 | Average % | 93 | ||
| Range % | 87-96 | |||
| 1:8 | Average % | 87 | ||
| Range % | 80-96 |
回收率：
| The recovery of human TrpV1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 96 | 89-99 | |||||||||||
| EDTA plasma (n=4) | 94 | 90-98 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| ng/mlOD1OD2AverageCorrected 201.9872.1492.0681.906 101.6331.5731.6031.441 51.1191.1371.1280.966 2.50.7740.7900.7820.620 1.250.4410.4610.4510.289 0.6250.3240.3060.3150.153 0.3120.2290.2310.2300.068 00.1610.1620.162 | |||||||||||||
货期：3-5 working days
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