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产品描述:人运甲状腺素蛋白/前白蛋白(TTR)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的TTR含量。TTR(转甲状腺素蛋白)是一种甲状腺激素结合蛋白,与甲状腺激素的运输有关。研究发现,TTR在阿尔茨海默病(AD)中具有神经保护作用,能够抑制β淀粉样蛋白的聚集,其作用机制涉及TTR与Aβ的相互作用,通过“扣押”Aβ单体来抑制其聚集,降低Aβ聚集体的细胞毒性。试剂盒检测范围为1.56 μg/mL-25 μg/mL,适用于科研领域中对TTR表达水平的研究,例如神经退行性疾病模型构建、代谢相关蛋白相互作用分析,或药物干预下TTR动态变化的评估。适用于基础医学研究、病理机制探索以及生物标志物筛选等科研场景。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Amyloid polyneuropathy ELISA Kit; Amyloidosis I ELISA Kit; ATTR ELISA Kit; Carpal tunnel syndrome 1 ELISA Kit; CTS ELISA Kit; CTS1 ELISA Kit; Dysprealbuminemic euthyroidal hyperthyroxinemia ELISA Kit; Dystransthyretinemic hyperthyroxinemia ELISA Kit; Epididymis luminal protein 111 ELISA Kit; HEL111 ELISA Kit; HsT2651 ELISA Kit; PALB ELISA Kit; Prealbumin amyloidosis type I ELISA Kit; Prealbumin ELISA Kit; Prealbumin Thyroxine-binding ELISA Kit; Senile systemic amyloidosis ELISA Kit; TBPA ELISA Kit; Thyroxine binding prealbumin ELISA Kit; Transthyretin ELISA Kit; TTHY_HUMAN ELISA Kit; TTR ELISA Kit; TTR protein ELISA Kit
缩写:TTR
Uniprot No.:P02766
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:1.56 μg/mL-25 μg/mL
灵敏度:0.78 μg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cardiovascular
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human TTR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:100 | Average % | 90 | ||||
Range % | 87-97 | |||||
1:200 | Average % | 97 | ||||
Range % | 93-104 | |||||
1:400 | Average % | 85 | ||||
Range % | 82-91 | |||||
1:800 | Average % | 90 | ||||
Range % | 85-97 |
回收率:
The recovery of human TTR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 89 | 85-93 | ||||
EDTA plasma (n=4) | 92 | 88-94 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
μg/mlOD1OD2Average 250.1650.1620.164 12.50.3110.3050.308 6.250.6120.6060.609 3.120.8560.8490.853 1.561.5831.5751.579 02.7152.6902.703 |
货期:3-5 working days
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