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产品描述：牛血管内皮生长因子A(VEGFA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VEGFA含量。VEGFA即血管内皮生长因子A，是一种促进血管生成的重要因子。其背景在于在肿瘤、眼科疾病等病理状态下表达常异常升高。研究机制主要是通过与受体结合，激活下游信号通路，促进血管内皮细胞的增殖、迁移，形成新生血管，为疾病发展提供营养支持。试剂盒检测范围为47 pg/mL-3000 pg/mL，本产品适用于牛VEGFA表达调控机制研究、心血管疾病模型评估、肿瘤血管生成实验及生殖生理相关科研项目，为兽医基础研究、畜牧业抗病育种及生物制药领域提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：VEGFA ELISA kit; VEGFVascular endothelial growth factor A ELISA kit; VEGF-A ELISA kit; Vascular permeability factor ELISA kit; VPF ELISA kit
缩写：VEGFA
Uniprot No.：P15691
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine VEGFA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 88102 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 87-92 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 84-96 | ||||||
回收率：
| The recovery of bovine VEGFA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 84-95 | |||||
| EDTA plasma (n=4) | 104 | 97-111 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected30002.1471.9882.0681.94015001.6041.5761.5901.4627501.1051.0891.0970.9693750.7560.7670.7620.634187.50.5380.5310.5350.407940.3400.3320.3360.208470.2280.2210.2250.09700.1270.1290.128 | |||||||
货期：3-5 working days
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