首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血管内皮细胞生长因子C(VEGF-C)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VEGFC含量。VEGFC即血管内皮生长因子C，在胚胎淋巴管发育中发挥重要作用，可刺激淋巴内皮细胞生长和迁移。其信号通路异常和肿瘤淋巴转移相关，研究机制多聚焦于该信号通路及在癌症治疗中的应用，有望为癌症淋巴转移治疗带来新思路。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研领域，如肿瘤生物学研究中转移机制探索、心血管疾病模型中的血管生成评估，以及药物开发过程中靶向治疗效能的体外检测，支持细胞培养上清、动物模型样本及临床前研究样本中 VEGF-C 蛋白表达水平的精准量化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Flt 4L ELISA Kit; Flt4 ligand ELISA Kit; FLT4 ligand DHM ELISA Kit; Flt4-L ELISA Kit; Flt4L ELISA Kit; Vascular endothelial growth factor C ELISA Kit; Vascular endothelial growth factor related protein ELISA Kit; Vascular endothelial growth factor-related protein ELISA Kit; VEGF C ELISA Kit; VEGF-C ELISA Kit; Vegfc ELISA Kit; VEGFC_HUMAN ELISA Kit; VRP ELISA Kit
缩写：VEGFC
Uniprot No.：P49767
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:1 | Average % | 100 | |||||||||||
| Range % | 96-105 | ||||||||||||
| 1:2 | Average % | 92 | |||||||||||
| Range % | 87-99 | ||||||||||||
| 1:4 | Average % | 95 | |||||||||||
| Range % | 90-101 | ||||||||||||
| 1:8 | Average % | 92 | |||||||||||
| Range % | 88-96 | ||||||||||||
回收率：
| The recovery of human VEGF-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 99 | 94-106 | |||||||||||
| EDTA plasma (n=4) | 90 | 84-96 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| pg/mlOD1OD2AverageCorrected10002.5752.5432.5592.4465001.8801.7761.8281.7152501.0511.0341.0430.9301250.6130.5870.6000.48762.50.3870.3710.3790.26631.20.2350.2260.2310.11815.60.1690.1580.1640.05100.1150.1110.113 | |||||||||||||
货期：3-5 working days
×
