| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VSNL1 |
| Uniprot No | P62760 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-191aa |
| 氨基酸序列 | MGKQNSKLAPEVMEDLVKSTEFNEHELKQWYKGFLKDCPSGRLNLEEFQQLYVKFFPYGDASKFAQHAFRTFDKNGDGTIDFREFICALSITSRGSFEQKLNWAFNMYDLDGDGKITRVEMLEIIEAIYKMVGTVIMMKMNEDGLTPEQRVDKIFSKMDKNKDDQITLDEFKEAAKSDPSIVLLLQCDIQK |
| 预测分子量 | 49.0kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VSNL1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Visinin-like protein 1 (VSNL1) as a potential biomarker for Alzheimer's disease: Expression and purification of recombinant VSNL1 in Escherichia coli"*
**作者**: Kumar A, et al.
**摘要**: 本研究报道了VSNL1重组蛋白在大肠杆菌中的高效表达和纯化方法，通过优化诱导条件获得可溶性蛋白，并验证其钙离子结合活性。该重组蛋白被用于开发阿尔茨海默病(AD)患者脑脊液中的VSNL1检测方法，表明其在AD早期诊断中的潜在应用价值。
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2. **文献名称**: *"Structural and functional analysis of recombinant human VSNL1: Insights into its role in neuronal calcium signaling"*
**作者**: Sánchez JC, et al.
**摘要**: 通过X射线晶体学解析了重组人源VSNL1的三维结构，揭示了其EF-hand结构域与钙离子的特异性结合模式。实验表明，重组VSNL1通过钙依赖机制调控神经元突触前膜的信号转导，为理解其在神经退行性疾病中的作用提供了结构基础。
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3. **文献名称**: *"VSNL1 recombinant protein modulates synaptic plasticity via interaction with postsynaptic density proteins"*
**作者**: Chen L, et al.
**摘要**: 研究利用重组VSNL1蛋白探究其对海马神经元突触可塑性的影响。结果显示，重组VSNL1通过结合突触后致密区蛋白(如PSD-95)调控NMDA受体活性，并影响长时程增强(LTP)过程，提示其在记忆相关病理机制中的关键作用。
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*注：以上文献信息为模拟生成，实际引用时需核实真实来源及内容准确性。如需真实文献，建议通过PubMed或Web of Science以“VSNL1 recombinant protein”为关键词检索。*
**Background of VSNL1 Recombinant Protein**
VSNL1 (Visinin-like protein 1), also known as VILIP-1. is a calcium-sensing protein belonging to the neuronal calcium sensor (NCS) family. It plays a critical role in regulating intracellular calcium signaling pathways, particularly in neurons, where it modulates synaptic plasticity, neurotransmitter release, and neuronal survival. Structurally, VSNL1 contains EF-hand motifs that bind Ca²⁺ ions, enabling it to act as a Ca²⁺-dependent switch for interacting with target proteins, such as membrane receptors and enzymes.
Dysregulation of VSNL1 has been implicated in neurodegenerative disorders, including Alzheimer’s disease (AD), where its expression is altered in early stages, potentially serving as a biomarker. Studies suggest that VSNL1 interacts with amyloid-beta (Aβ) and tau proteins, contributing to pathological cascades. Additionally, VSNL1 is linked to cancer progression, as it influences cell proliferation and metastasis in certain malignancies.
Recombinant VSNL1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research applications. It serves as a tool to study Ca²⁺-mediated signaling mechanisms, screen therapeutic compounds, or validate diagnostic markers. Its recombinant form retains native conformation and activity, enabling *in vitro* and *in vivo* studies to elucidate its physiological roles and disease associations. Ongoing research aims to harness VSNL1’s regulatory functions for developing targeted therapies in neurology and oncology.
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