首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人玻连蛋白(VTN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VTN含量。VTN靶点指血管转铁蛋白，参与免疫调节等生理过程。研究发现其与肿瘤免疫逃逸相关，阻断VTN可激活机体抗肿瘤免疫反应。目前围绕该靶点开发的治疗方法，有望为肿瘤治疗带来新策略，不过其具体机制仍待深入研究。试剂盒检测范围为1.56 ng/mL-100 ng/mL，科研人员可通过该产品研究VTN在肝脏纤维化、动脉粥样硬化、肿瘤转移等疾病模型中的表达动态，探索其与炎症因子互作机制，或作为生物标志物参与病理过程评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Complement S Protein ELISA Kit; Epibolin ELISA Kit; S Protein ELISA Kit; S-protein ELISA Kit; Serum Spreading Factor ELISA Kit; Serum-spreading factor ELISA Kit; Somatomedin B ELISA Kit; Somatomedin-B ELISA Kit; V75 ELISA Kit; Vitronectin ELISA Kit; Vitronectin V10 subunit ELISA Kit; Vitronectin V65 subunit ELISA Kit; VN ELISA Kit; VNT ELISA Kit; VTN ELISA Kit; VTNC_HUMAN ELISA Kit
缩写：VTN
Uniprot No.：P04004
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 88-99 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 95-105 | |||||
回收率：
| The recovery of human VTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 95 | 92-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1002.4872.4992.4932.369 502.0122.0572.0351.911 251.3561.3181.3371.213 12.50.7350.7210.7280.604 6.250.4990.4840.4920.368 3.120.3460.3240.3350.211 1.560.2210.2350.2280.104 00.1230.1250.124 | ||||||
货期：3-5 working days
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