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产品描述：人Wnt-2蛋白(WNT2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的WNT2含量。WNT2是一种重要的靶点。作为Wnt蛋白家族成员，其参与调控细胞生长、发育等过程。研究主要聚焦于它在信号通路中的作用机制，如Wnt/β -连环蛋白信号通路。异常激活或表达可能与肿瘤等疾病发生发展相关，为相关疾病治疗提供潜在方向。试剂盒检测范围为0.45 ng/mL-30 ng/mL，适用于生命科学研究领域，可用于探索Wnt信号通路相关疾病模型中的蛋白表达特征、验证体外细胞实验的分泌水平变化或评估特定干预条件下生物样本的WNT2动态表达，为分子机制研究提供定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Int 1 related protein ELISA Kit; Int-1-like protein 1 ELISA Kit; Int-1-related protein ELISA Kit; INT1L 1 ELISA Kit; INT1L1 ELISA Kit; IRP ELISA Kit; IRP protein ELISA Kit; ONCOGENE INT1 LIKE 1 ELISA Kit; Protein Wnt-2 ELISA Kit; Secreted growth factor ELISA Kit; Wingless type MMTV integration site family member 2 ELISA Kit; WNT 2 ELISA Kit; WNT2 ELISA Kit; WNT2_HUMAN ELISA Kit
缩写：WNT2
Uniprot No.：P09544
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.45 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human WNT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:4 | Average % | 105 | ||||
| Range % | 98-109 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of human WNT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-98 | ||||
| EDTA plasma (n=4) | 88 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected302.5242.6342.5792.466151.8961.7561.8261.7137.51.1811.1621.1721.0593.750.7190.7380.7290.6161.80.4090.4240.4170.3040.90.2970.2860.2920.1790.450.2100.2150.2130.10000.1120.1130.113 | ||||||
货期：3-5 working days
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