首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠黄嘌呤氧化酶(XOD)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的XDH含量。XDH即黄嘌呤脱氢酶，在嘌呤代谢中起着关键作用，可催化黄嘌呤转化为尿酸。异常的XDH活性与高尿酸血症、痛风等疾病密切相关。研究通常聚焦于其结构和功能，开发能调节该靶点活性的药物以治疗相关病症。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本试剂盒基于高特异性抗体对目标蛋白的捕获与检测，适用于科研场景中探究XOD在疾病模型中的表达水平变化，或用于评估药物干预、营养调控对嘌呤代谢通路的影响。样本兼容性强，可满足血清、血浆等液体样本及多种组织匀浆的检测需求，为氧化应激相关基础研究和代谢机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Xdh ELISA Kit; Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase ELISA Kit; XD ELISA Kit; EC 1.17.1.4); Xanthine oxidase ELISA Kit; XO ELISA Kit; EC 1.17.3.2 ELISA Kit; Xanthine oxidoreductase ELISA Kit; XOR)] ELISA Kit
缩写：XDH
Uniprot No.：P22985
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat XOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 96-105 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of rat XOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-104 | ||||
| EDTA plasma (n=4) | 104 | 96-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.7112.6102.6612.544 102.2082.1072.1582.041 51.5071.4861.4971.380 2.50.9090.8980.9040.787 1.250.4670.4560.4620.345 0.6250.2800.2910.2860.169 0.3120.1850.1880.1870.070 00.1160.1170.117 | ||||||
货期：3-5 working days
×
