首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠β内啡肽(β-EP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的β-EP含量。β-EP即β-内啡肽，是内源性阿片肽家族中的重要成员，广泛分布于中枢神经系统和外周组织。其研究机制主要围绕它与阿片受体结合，在痛觉调制、神经内分泌调节、情绪调控等方面发挥关键作用，对了解机体生理和病理过程意义重大。试剂盒检测范围为0.78 pg/mL-50 pg/mL，适用于基础科研领域，如探究中枢神经系统疾病模型、应激反应机制或镇痛药物研究中β-EP的动态变化，亦可用于评估动物模型中组织特异性β-EP表达水平。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：beta-Endorphin, beta-EP
缩写：β-EP
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 pg/mL-50 pg/mL
灵敏度：0.195 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 86-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 97-104 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 89-95 | |||||
回收率：
| The recovery of mouse β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-98 | ||||
| EDTA plasma (n=4) | 94 | 90-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected502.2842.2982.2912.099251.7821.7881.7851.59312.51.1411.1691.1550.9636.250.6380.6010.6200.4283.120.4590.4360.4480.2561.560.3690.3420.3560.1640.780.2980.2840.2910.09900.1990.1850.192 | ||||||
货期：3-5 working days
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