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产品描述:人β血小板球蛋白/β血栓环蛋白(β-TG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的β-TG含量。β-TG即β-血小板球蛋白,是血小板α颗粒特有的蛋白质。其释放水平可反映血小板的活化程度。研究中常用它作为体内血小板激活的指标,探究其在血栓形成、炎症反应等病理过程中的作用及机制,以寻找相关疾病诊断和治疗的新靶点。试剂盒检测范围为0.78 ng/mL-50 ng/mL,本试剂盒适用于基础科研中对血小板功能评估、血栓性疾病机制研究、药物干预效果分析等场景,例如通过检测患者血浆中β-TG浓度变化探索抗血小板药物的作用机理,或利用组织匀浆分析β-TG在局部病理微环境中的表达特征本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:beta-Thromboglobulin, beta-TG
缩写:β-TG
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:0.78 ng/mL-50 ng/mL
灵敏度:0.195 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Others
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human β-TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 92 | ||||
Range % | 88-97 | |||||
1:2 | Average % | 90 | ||||
Range % | 85-95 | |||||
1:4 | Average % | 103 | ||||
Range % | 97-109 | |||||
1:8 | Average % | 84 | ||||
Range % | 80-89 |
回收率:
The recovery of human β-TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 97 | 92-101 | ||||
EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected502.7422.8212.7822.663251.9062.0151.9611.84212.51.2031.2741.2391.1206.250.6570.7030.6800.5613.120.4350.4040.4200.3011.560.3250.3190.3220.2030.780.2050.1910.1980.07900.1220.1150.119 |
货期:3-5 working days
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