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产品描述:兔子β血小板球蛋白/β血栓环蛋白(β-TG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的β-TG含量。试剂盒检测范围为12.5 ng/mL-800 ng/mL,适用于血小板功能评估、血栓形成机制研究、心血管疾病模型病理过程探索等科研领域。为体外研究β - TG在止血、炎症反应及血管内皮损伤中的生物学作用提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:beta-Thromboglobulin, beta-TG
缩写:β-TG
种属:Oryctolagus cuniculus (Rabbit)
样本类型:serum, plasma, tissue homogenates
检测范围:12.5 ng/mL-800 ng/mL
灵敏度:3.12 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Others
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays):CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit β-TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 91 | |||||
Range % | 86-95 | ||||||
1:2 | Average % | 102 | |||||
Range % | 97-107 | ||||||
1:4 | Average % | 91 | |||||
Range % | 85-97 | ||||||
1:8 | Average % | 97 | |||||
Range % | 91-103 |
回收率:
The recovery of rabbit β-TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 95 | 89-98 | |||||
EDTA plasma (n=4) | 97 | 90-100 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2AverageCorrected8002.8012.8692.8352.7484002.4842.3982.4412.3542001.7161.7681.7421.6551000.9440.9680.9560.869500.4540.4680.4610.374250.2560.2480.2520.16512.50.1470.1510.1490.06200.0850.0890.087 |
货期:3-5 working days
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