首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠髓过氧化物酶特异性抗中性粒细胞胞质抗体(MPO-ANCA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的MPO-ANCA含量。MPO-ANCA（髓过氧化物酶特异性抗中性粒细胞胞浆抗体）是一种与血管炎和肾小球肾炎密切相关的自身抗体。其研究机制主要涉及MPO分子被自身免疫性T/B细胞识别的抗原表位，以及易感HLA分子、补体活化、凝血和血小板等参与的免疫炎症反应。MPO-ANCA的致病性在动物模型和临床研究中得到证实，其与疾病活动度密切相关，是治疗的关键靶点。试剂盒检测范围为0.625 U/mL-40 U/mL，该产品适用于科研场景中血管炎动物模型的抗体水平动态监测、疾病机制探索以及药物干预效果评价等研究，为深入解析MPO - ANCA在免疫病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：MPO-ANCA
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：0.625 U/mL-40 U/mL
灵敏度：0.156 U/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MPO-ANCA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-95 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of rat MPO-ANCA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 91 | 84-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| U/mlOD1OD2AverageCorrected402.6122.7522.6822.518202.0132.0762.0451.881101.3671.3041.3361.17250.8020.8370.8200.6562.50.4440.4240.4340.2701.250.3510.3590.3550.1910.6250.2410.2250.2330.06900.1600.1670.164 | ||||||
货期：3-5 working days
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