首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠高半胱氨酸(Hcy)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Hcy含量。Hcy即同型半胱氨酸，是蛋氨酸代谢的中间产物。其水平升高是心脑血管疾病等多种疾病的独立危险因素。研究机制上，可能通过损伤血管内皮细胞、促进血栓形成、氧化应激等途径参与疾病发生发展，对其深入研究有重要临床意义。试剂盒检测范围为0.78 nmol/mL-50 nmol/mL，广泛应用于大鼠模型的基础研究，例如在高血压、动脉粥样硬化或阿尔茨海默病等动物模型中评估Hcy水平变化，或用于探究营养干预、药物代谢对Hcy调控的影响，为疾病机制解析及治疗策略开发提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：Hcy
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 nmol/mL-50 nmol/mL
灵敏度：0.195 nmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 93-103 | |||||
回收率：
| The recovery of rat Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-97 | ||||
| EDTA plasma (n=4) | 95 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| nmol/mlOD1OD2AverageCorrected502.8742.7712.8232.670252.2232.2452.2342.08112.51.4181.3891.4041.2516.250.7560.7750.7660.6133.120.4620.4480.4550.3021.560.3210.3130.3170.1640.780.2320.2250.2290.07600.1550.1510.153 | ||||||
货期：3-5 working days
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