首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胰淀粉酶α2A(AMY2A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AMY2A含量。AMY2A是一个靶点。它主要编码胰腺α -淀粉酶，在碳水化合物消化中起着关键作用。目前研究围绕其与碳水化合物代谢相关机制展开，探究其功能异常与代谢疾病如糖尿病等的关联，期望通过调节该靶点来改善相关病症。试剂盒检测范围为3.12 mIU/mL-200 mIU/mL，该产品适用于科研领域中对大鼠胰腺功能的基础研究，如评估营养代谢干预、胰腺炎模型或糖尿病等代谢性疾病动物模型的病理机制，也可用于药物开发中胰腺相关生物标志物的动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：AMY2A
Uniprot No.：P00689
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 mIU/mL-200 mIU/mL
灵敏度：0.78 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AMY2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 84-93 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 94-105 | |||||
回收率：
| The recovery of rat AMY2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-101 | ||||
| EDTA plasma (n=4) | 92 | 88-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected 2002.6272.5742.6012.497 1001.9851.8521.9191.815 501.1041.1261.1151.011 250.6280.6090.6190.515 12.50.3600.3810.3710.267 6.250.2590.2470.2530.149 3.120.1810.1740.1780.074 00.1030.1050.104 | ||||||
货期：3-5 working days
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