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产品描述：鸡的牛血清白蛋白(BSA) 残留检测酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BSA含量。试剂盒检测范围为0.312 μg/mL-80 μg/mL，灵敏度为0.312 μg/mL。适用于科研领域中对生物制品、疫苗生产或实验样本处理流程中BSA污染物的质量控制；适用于评估样本纯化工艺效果、细胞培养基质残留分析或生物制药流程中的BSA监控；助力科研人员优化实验体系并保障数据可靠性本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：BSA
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 μg/mL-80 μg/mL
灵敏度：0.312 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken BSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2 | Average % | 106 | ||||
| Range % | 100-112 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of chicken BSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 800.2520.2470.250 200.4360.4620.449 50.8070.8540.831 1.251.3051.2571.281 0.3121.8791.9241.902 02.6012.5592.580 | ||||||
货期：3-5 working days
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