首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胱天蛋白酶9(Casp-9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CASP9含量。CASP9是一种半胱天冬酶，在细胞凋亡机制中扮演核心角色。其作为关键调节因子，通过激活级联反应诱导细胞程序性死亡。目前对它的研究主要围绕深入解析凋亡途径、探索其与疾病关联及开发以它为靶点的治疗手段，助力攻克相关疾病。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于基础科研领域中对细胞凋亡机制、药物干预效应或疾病模型中Caspase - 9动态变化的定量分析，可为体外实验、动物模型或细胞水平研究提供可靠的检测数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：APAF-3 ELISA Kit; Apoptotic protease Mch-6 ELISA Kit; Apoptotic protease-activating factor 3 ELISA Kit; CASP-9 ELISA Kit; CASP9 ELISA Kit; CASP9_HUMAN ELISA Kit; Caspase 9 ELISA Kit; Caspase-9 subunit p10 ELISA Kit; ICE-LAP6 ELISA Kit; ICE-like apoptotic protease 6 ELISA Kit
缩写：CASP9
Uniprot No.：P55211
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Casp-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 84-94 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 87-94 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 94-103 | |||||
回收率：
| The recovery of human Casp-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 88-97 | ||||
| EDTA plasma (n=4) | 105 | 97-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.7022.5462.6242.520251.5261.5331.5301.42612.50.7450.7640.7550.6516.250.4790.4860.4830.3793.120.2890.2930.2910.1871.560.2120.2150.2140.1100.780.1610.1640.1630.05900.1060.1020.104 | ||||||
货期：3-5 working days
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