关键信息
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基因名
SDHA
- 应用
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别名
SDH2; SDHF; FP; Flavoprotein; Flavoprotein subunit of complex II; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
-
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表达区间
Val4~Ile300
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分子量
36kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Succinate dehydrogenase (SDH) is a critical enzyme complex involved in the mitochondrial respiratory chain and the tricarboxylic acid (TCA) cycle, playing a key role in cellular energy production and metabolism. Dysregulation of SDH is associated with various pathologies, including hereditary paraganglioma, pheochromocytoma, and specific types of cancer, making it a significant target for biomedical research. The study of recombinant SDHA (the flavoprotein subunit of SDH) is particularly important for understanding its structural and functional properties, as well as its interactions with other subunits and metabolites. Producing recombinant SDHA allows for detailed biochemical studies, facilitating the exploration of enzyme kinetics, substrate specificity, and response to inhibitors, thus enhancing our knowledge of its role in health and disease. Furthermore, investigating SDHA mutations can elucidate their impact on enzyme functionality, contributing to the development of therapeutic strategies for related disorders. As such, SDHA recombinant protein research not only aids in understanding fundamental biological processes but also holds potential for translational applications in disease treatment and prevention.
