首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人免疫球蛋白j链(Ig-j)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、唾液、尿液样本中的IGJ含量。IGJ即免疫球蛋白J链，它参与分泌型免疫球蛋白的组装与转运。在研究机制上，其可调节免疫球蛋白多聚化，影响黏膜免疫功能。对IGJ的深入研究有助于理解免疫相关疾病发病机制，为开发针对免疫调节的治疗方法提供理论支持。试剂盒检测范围为10 ng/mL-40000 ng/mL，适用于科研领域中对黏膜免疫机制、感染性疾病或免疫缺陷相关研究，也可用于评估母乳喂养中免疫成分传递效率、泪液或唾液局部免疫状态分析等实验场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IGCJ ELISA Kit; IGJ_HUMAN ELISA Kit; Immunoglobulin J chain ELISA Kit; Immunoglobulin J polypeptide linker protein for immunoglobulin alpha and mu polypeptides ELISA Kit; immunoglobulin joining chain ELISA Kit; JCH ELISA Kit; JCHAIN ELISA Kit; Joining chain of multimeric IgA and IgM ELISA Kit
缩写：IGJ
Uniprot No.：P01591
种属：Homo sapiens (Human)
样本类型：serum, plasma, saliva, urine, tear, breast milk
检测范围：10 ng/mL-40000 ng/mL
灵敏度：10 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays):CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IGJ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1000 | Average % | 89 | |||||
| Range % | 81-99 | ||||||
| 1:2000 | Average % | 95 | |||||
| Range % | 83-99 | ||||||
| 1:4000 | Average % | 95 | |||||
| Range % | 90-102 | ||||||
| 1:8000 | Average % | 90 | |||||
| Range % | 85-100 | ||||||
回收率：
| The recovery of human IGJ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 85-99 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 400002.2572.2422.2502.162 100002.1542.1382.1462.058 25001.8921.8641.8781.790 6251.2521.2491.2511.163 1560.5870.5980.5930.505 390.2820.2880.2850.197 100.1410.1390.1400.052 00.0860.0900.088 | |||||||
货期：3-5 working days
×
